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Tucker Prairie is a 146-acre tract of virgin tall grass prairie in Callaway County, Missouri. This tract of land is the largest prairie remnant in mid-Missouri. It has never been plowed. The tract is dominated by the typical tall grass prairie species, including big bluestem, little bluestem, Indian grass, and prairie dropseed, but supports more than 250 species of plants. Tucker Prairie has an extensive subterranean clay pan that makes tree growth difficult, thus enhancing its unique qualities.

The prairie is managed by the Division of Biological Sciences at the University of Missouri in cooperation with the Missouri Department of Conservation. It is used as a research tract principally by faculty and students of the University of Missouri but outside investigators are welcome.

Anyone wishing to conduct research or classes on Tucker Prairie must complete and submit a permit application.